Gst expression manual

E. coli cold shock gene. Thus, the pCold expression system can significantly improve protein expression, purity, and solubility1). The expression vector pCold GST DNA was developed by incorporating glutathione S-transferase (GST) derived from Schistosoma japonicum as a soluble tag2), 3). The proteins. GST Tag ELISA Detection Kit Cat. No. L Technical Manual No. TM Version Genscript GST Tag ELISA Detection Kit is used for the detection of GST tagged protein in samples. Checking GST tagged protein expression and . Recombinant Protein Expression Purification 11 Gene Fusion Expression Systems Glutathione S-transferase (GST) pGEX Vectors*(GST Gene fusion) All of the GST gene fusion vectors offer: • A tac promoter for chemically inducible, high-level expression. Map of the glutathione S-transferase fusion vectors showing the reading frames and main features.

Protocol # PT www.doorway.ru Clontech Laboratories, Inc. Version # PR74 30 3 Glutathione Resins User Manual I. Introduction Epitope tags such as GST are often used to label proteins for expression and. The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the system. Recombinant Protein Expression Purification 11 Gene Fusion Expression Systems Glutathione S-transferase (GST) pGEX Vectors*(GST Gene fusion) All of the GST gene fusion vectors offer: • A tac promoter for chemically inducible, high-level expression. Map of the glutathione S-transferase fusion vectors showing the reading frames and main features.

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